mouse recombinant epo  (R&D Systems)

Journal: Leukemia

Article Title: HDAC7 is a potential therapeutic target in acute erythroid leukemia

doi: 10.1038/s41375-024-02394-5

Figure Lengend Snippet: A Experimental scheme used in ( B – D ). Human cord blood (CB) CD34 + cells were transduced with p53DD (coexpressing NGFR) and ERG (coexpressing GFP) and cultured in the presence of EPO. B Proliferation curves of CB cells transduced with vector, p53DD, ERG, or p53DD+ERG for 4 days. Results are expressed as mean ± s.e.m. of three independent experiments. **** P < 0.0001. CD71 and CD235a expression ( C ) and Wright-Giemsa-staining ( D ), original magnification x400) of CB cells transduced with vector, p53DD, ERG, or p53DD+ERG. E CD235a expression of F36P, HEL, and TF-1 cells transduced with vector or ERG.

Article Snippet: CEP53 cells were cultured in RPMI-1640 medium supplemented with 10% FBS containing 1 ng/ml EPO (#959-ME, R&D Systems).

Techniques: Transduction, Cell Culture, Plasmid Preparation, Expressing, Staining

Journal: Cell Death & Disease

Article Title: A novel function of STAT3β in suppressing interferon response improves outcome in acute myeloid leukemia

doi: 10.1038/s41419-024-06749-9

Figure Lengend Snippet: A FLCs from WT and STAT3β–deficient (Δβ) mice were retrovirally transformed using a vector encoding MLL-AF9 . B Western blot analysis of MLL-AF9 transformed FLCs with the indicated antibodies. C Growth curves of MLL-AF9 transformed FLCs (n = 6, 3 independent experiments, 2 cell lines). D Colony formation assay of WT and STAT3β-deficient leukemia cells (n = 8, 3 independent experiments with 3 serial replatings). E Representative pictures of colonies after 7 days in methylcellulose (40× magnification). F Immunophenotyping of leukemia cells after 7 days in methylcellulose via flow cytometry and representative dot plots (n = 8, 3 experiments, 3 serial replatings). Statistical analysis was performed using Student’s t test and indicated as p ≤ 0.05:*, ≤0.01:**. Error bars represent means ± SD.

Article Snippet: 1×10 4 cells were seeded in 1 ml methylcellulose with recombinant cytokines (SCF, IL-3, IL-6, and EPO) for mouse cells (MethoCult TM GF M3434, Stemcell Technologies, Vancouver, Canada) in 35 mm cell culture dishes and incubated at 37 °C, 95% humidity and 5% CO 2 .

Techniques: Transformation Assay, Plasmid Preparation, Western Blot, Colony Assay, Flow Cytometry

Journal: Cell Death & Disease

Article Title: A novel function of STAT3β in suppressing interferon response improves outcome in acute myeloid leukemia

doi: 10.1038/s41419-024-06749-9

Figure Lengend Snippet: A mRNA expression after 3 h 100 U/ml IFNβ stimulation (n = 8, 3 independent experiments, 2–3 cell lines). B mRNA expression after 3 h 100 ng/ml IFNγ stimulation (n = 9, 3 independent experiments, 3 cell lines). C Western blot after 24 h IFN treatment. D Flow cytometry quantification of CD11b + and Sca-1 + cells after 48 h IFNγ treatment (n = 6, 3 independent experiments, 2 cell lines). E Heatmap represents the average concentration measured in the plasma of diseased animals via multiplex assay. (n = 5 per group, 3 controls). F mRNA expression of Ifna, Ifnb, and Ifng in leukemia cells after 7 days in methylcellulose analyzed via RT-qPCR (n = 5, 3 independent experiments, 2 cell lines). Statistical analysis was performed using Student’s t test and indicated as p ≤ 0.05:*, ≤0.01:**. Error bars represent means ± SD.

Article Snippet: 1×10 4 cells were seeded in 1 ml methylcellulose with recombinant cytokines (SCF, IL-3, IL-6, and EPO) for mouse cells (MethoCult TM GF M3434, Stemcell Technologies, Vancouver, Canada) in 35 mm cell culture dishes and incubated at 37 °C, 95% humidity and 5% CO 2 .

Techniques: Expressing, Western Blot, Flow Cytometry, Concentration Assay, Clinical Proteomics, Multiplex Assay, Quantitative RT-PCR

Journal: Cell Death & Disease

Article Title: A novel function of STAT3β in suppressing interferon response improves outcome in acute myeloid leukemia

doi: 10.1038/s41419-024-06749-9

Figure Lengend Snippet: A Colonies formed in the presence of 2 µg/ml IFNAR1 blocking antibody normalized to untreated (n = 8 from 3 independent experiments, 3 cell lines). B Representative images of colonies formed after 7 days in methylcellulose ± IFNAR1 blocking antibody or IgG control. C Colonies formed in the presence of 5 µM Ruxolitinib normalized to untreated (n = 6, 3 independent experiments, 2 cell lines). D Representative images of colonies formed ± Ruxolitinib or DMSO control. Statistical analysis was performed using Student’s t test and indicated as p ≤ 0.05:*. Error bars represent means ± SD.

Article Snippet: 1×10 4 cells were seeded in 1 ml methylcellulose with recombinant cytokines (SCF, IL-3, IL-6, and EPO) for mouse cells (MethoCult TM GF M3434, Stemcell Technologies, Vancouver, Canada) in 35 mm cell culture dishes and incubated at 37 °C, 95% humidity and 5% CO 2 .

Techniques: Blocking Assay, Control